| 摘要 |
To study different types of thrombolytic agents, both thrombolysis and stimulation of fucoidan were studied in a mouse model of ferric chloride-induced arterial thrombosis and were compared with those of a heparin and tissue plasminogen activator (t-PA). Thrombosis was induced by applying a filter paper saturated with 5% FeCl3 to the left carotid artery. 20 minutes after complete occlusion, several test preparations including a fucoidan source from Undaria pinnatifida sporophylls were intravenously injected to each mouse group by various dosages of 10-200 mg/kg-1. To study the stimulation effect of fucoidan with respect to t-PA, fucoidan added in a predetermined amount of t-PA was injected by a dose of 1-10 mg/kg. Then, the time to reperfusion was measured. As a result, an occluded aorta was dose-dependently reperfused, and the reperfusion was observed at 37.5±12.4 minutes after the administration of 100 mg/kg-1 of unfractionated fucoidan. The time to reperfusion was retarded to 55.0±8.0 minutes when a large amount of fucoidan such as 1 g/kg-1 was administered to a mouse provided with low molecular weight fucoidan (LMWF). Reperfusion occurred at 16.67 minutes after administration in a mouse of a control group provided with 20 mg/kg-1 of t-PA. On the contrary, reperfusion was not observed in an occluded mouse provided with heparin (p<0.01). The occluded artery was recovered without reperfusion at 17.2 minutes after injection if the least amount of fucoidan added in a predetermined amount of t-PA was provided. All the data show that fucoidan can serve as both a thrombolytic agent and a stimulant with respect to thrombolysis activation of t-PA in a thrombosis model, differently from heparin. |
