发明名称 |
MICRORNA AFFINITY ASSAY AND USES THEREOF |
摘要 |
The present invention provides methods and kits for determining which microRNAs bind to a target mRNA where the methods comprise the steps of (a) creating a bait sequence from the target mRNA, where the bait sequence comprises a label that binds to a binding agent; (b) adding a mixture of microRNAs to the bait sequence; (c) separating the microRNAs that bind to the bait sequence from those microRNAs that do not bind; and (d) identifying the microRNAs that bind to the bait sequence, wherein the microRNAs identified are those that bind to the target mRNA. |
申请公布号 |
US2012264619(A1) |
申请公布日期 |
2012.10.18 |
申请号 |
US201013500083 |
申请日期 |
2010.10.18 |
申请人 |
SPIVACK SIMON D.;SHI MIAO;HAN WEIGUO |
发明人 |
SPIVACK SIMON D.;SHI MIAO;HAN WEIGUO |
分类号 |
C40B20/00;C12Q1/68;C40B60/10;G01N33/53 |
主分类号 |
C40B20/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|