This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in Thermobifida fusca Cel9A enzyme. In certain embodiments the glycoside hydrolase is a variant of a family 9 glycoside hydrolase. In certain embodiments the glycoside hydrolase is a variant of a theme B family 9 glycoside hydrolase.
申请公布号
WO2012142171(A2)
申请公布日期
2012.10.18
申请号
WO2012US33138
申请日期
2012.04.11
申请人
ALLOPARTIS BIOTECHNOLOGIES, INC.;COHEN, RICHARD N.;KOPPEL, NITZAN;TORIELLO, NICHOLAS M.;BLAZEJ, ROBERT G.;EMRICH, CHARLES A.
发明人
COHEN, RICHARD N.;KOPPEL, NITZAN;TORIELLO, NICHOLAS M.;BLAZEJ, ROBERT G.;EMRICH, CHARLES A.