发明名称 |
LENTIVIRAL-BASED VECTOR AND ITS USE IN DIRECTED EVOLUTION OF GENOMIC REGIONS, GENES AND POLYNUCLEOTIDES |
摘要 |
The present invention concerns a method of directing evolution of a target polynucleotide of interest for obtaining variants of this target polynucleotide, as well as a method to generate genetic variability by preparing a cell library. This invention also relates to a method to isolate or to screen variants of a polynucleotide or variants of a protein able to impact the phenotype of a cell or to confer a desired phenotype to target cells, and to identify theses polynucleotide variants or protein variants responsible for this phenotype.
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申请公布号 |
US2012264616(A1) |
申请公布日期 |
2012.10.18 |
申请号 |
US201013509027 |
申请日期 |
2010.11.10 |
申请人 |
NEGRONI MATTEO;GALLOIS-MONTBRUN SARAH;ROSSOLILLO PAOLA;DI BARTOLO VINCENZO;UZE GILLES;SIMON-LORIERE ETIENNE;MARQUET ROLAND;VIVET-BOUDOU VALERIE;UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6);CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE |
发明人 |
NEGRONI MATTEO;GALLOIS-MONTBRUN SARAH;ROSSOLILLO PAOLA;DI BARTOLO VINCENZO;UZE GILLES;SIMON-LORIERE ETIENNE;MARQUET ROLAND;VIVET-BOUDOU VALERIE |
分类号 |
C40B20/00;C12N5/10;C12N7/01;C12N7/08;C12N15/49;C12N15/867;C40B40/02;C40B50/06 |
主分类号 |
C40B20/00 |
代理机构 |
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代理人 |
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