TECHNIQUES FOR BUFFERLESS LYSING OF CELLS AND SEPARATION OF CELLULAR COMPONENTS USING MODIFIED MEMBRANES

摘要

A porous membrane for lysis of a cell population enriched from a biological sample, and isolation of cellular components is provided. The porous membrane contains embedded lysing agents to perform lysing. The biological sample is brought into contact with the membrane. Lysis occurs through the action of the embedded lysing agents on the biological sample. The pores of the porous membrane are designed to have dimensions to allow only a desired type of cellular component(s) resulting from lysis to pass through the membrane, thereby achieving isolation of the desired cellular component(s). The action of lysing agents is combined with the filtration properties of porous membranes resulting in an easy-to-use and cost-effective technique.