METHOD OF DECIDING DETACHMENT TIME FOR CELL, METHOD OF SUBCULTURING CELL AND APPARATUS FOR SUBCULTURING CELL USING THE SAME

摘要

A method of deciding detachment time of cells, and a method and apparatus for subculturing the cells using the method. The method of deciding detachment time of the cells from a culturing vessel using a separation enzyme in subculturing the cells includes: a photographing step of photographing an image of the cells treated with the enzyme; a computing step of calculating a contrast value of the photographed image, and computing a change rate of the contrast value; a comparison step of repeating the photographing step and the computing step until an increasing change rate of the contrast value is reduced to a predetermined change rate or less; and a reporting step of reporting a time to remove the enzyme from the cells and to detach the cells from the culturing vessel when the increasing change rate of the contrast value is reduced to the predetermined change rate or less.