METHOD FOR IMPROVING STABILITY OF FLAVIN ADENINE DINUCLEOTIDE-DEPENDENT GLUCOSE DEHYDROGENASE

摘要

PROBLEM TO BE SOLVED: To provide a modified flavin adenine-dependent glucose dehydrogenase improved in stability by overcoming defects of a known flavin adenine-dependent glucose dehydrogenase.

SOLUTION: The modified FADGDH improved in stability is obtained by substituting an amino acid sequence in FADGDH with another amino acid by protein engineering method. A protein in which an amino acid at a position 339 in a specific amino acid sequence is substituted with another amino acid is provided. Alternatively, in the amino acid sequence having an amino acid sequence having ≥60% homology with at least either one of specific sequences and encoding a protein having glucose dehydrogenase activity, a protein in which the amino acid at the position 339 in the specific sequence is substituted with another amino acid is also provided.

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