| 摘要 |
Chondroitin sulfate proteoglycans are axon growth inhibitory molecules present in the glial scar that are responsible (at least in part) for regeneration failure after CNS or spinal cord injury. Removal of chondroitin sulfate glycosaminoglycan chains using the bacterial enzymes chondroitinase-ABC or AC in models of CNS injury promotes both axon regeneration and plasticity. The present invention relates to the use of mutants of human hyaluronidases and especially human hyaluronidase PH-20 (members of the human hyaluronidase family, endo-beta-acetyl-hexosaminidase enzymes, E.C. 3.2.1.35) with increased chondroitinase activity for the degradation of chondroitin sulfate (proteoglycans) in the glial scar to promote axonal regrowth in human CNS or spinal cord injury. |
