SSO7-POLYMERASE CONJUGATES WITH DECREASED NON-SPECIFIC ACTIVITY

摘要

Improved Sso7-polymerase conjugate proteins are provided. The present invention provides for an Ss07 polymerase conjugate protein comprising an Ss07 domain linked to a polymerase; wherein: an amino acid of the Ss07 domain corresponding to K28 of SEQ 10 NO:2 is not lysine (K); and/or an amino acid of the Ss07 domain corresponding to R43 of SEQ ID NO:2 is not arginine (R), wherein the conjugate protein has a decreased non-specific amplification activity compared to an otherwise identical control conjugate protein in which the amino acid of the Ss07 domain corresponding to K28 of SEQ ID NO:2 is lysine (K) and the amino acid of the Ss07 domain corresponding to R43 of SEQ ID NO:2 is arginine (R).