发明名称 |
TRANSFORMED HUMAN PLURIPOTENT STEM CELLS AND ASSOCIATED METHODS |
摘要 |
The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described.
|
申请公布号 |
US2012252697(A1) |
申请公布日期 |
2012.10.04 |
申请号 |
US201013393475 |
申请日期 |
2010.09.01 |
申请人 |
MCMASTER UNIVERSITY |
发明人 |
BHATIA MICKIE;WERBOWETSKI-OGILVIE TAMRA;SACHLOS ELEFTHERIOS;FISCHER RUSSELL DANIELA;LARONDE SARAH;LEE JUNGBOK;SZABO EVA;RISUENO RUTH M. |
分类号 |
C40B30/06;C12N5/0735;G01N21/64 |
主分类号 |
C40B30/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|