发明名称 |
Methods and Compositions for Enriching Either Target Polynucleotides or Non-Target Polynucleotides from a Mixture of Target and Non-Target Polynucleotides |
摘要 |
Compositions and methods are provided for enriching non-target polynucleotides from a mixture of non-target and target polynucleotides where differences between the target polynucleotides and the non-target polynucleotides include the extent of modified bases that are present in a greater density in the target polynucleotides than in the non-target polynucleotides. This permits the target polynucleotides to be selectively and rapidly bound to an affinity matrix such as affinity protein-coated magnetic beads providing enrichment of the non-target polynucleotides in the supernatant. One use of this enrichment is to remove human genomic DNA from a mixture of DNAs obtained from human tissue samples to enrich for polynucleotides in a microbiome so as to characterize the microbiome by DNA sequencing. |
申请公布号 |
US2012252009(A1) |
申请公布日期 |
2012.10.04 |
申请号 |
US201213435590 |
申请日期 |
2012.03.30 |
申请人 |
FEEHERY GEORGE R.;STEWART FIONA;MCFARLAND JAMES;PRADHAN SRIHARSA;NEW ENGLAND BIOLABS, INC. |
发明人 |
FEEHERY GEORGE R.;STEWART FIONA;MCFARLAND JAMES;PRADHAN SRIHARSA |
分类号 |
C12Q1/68;C07H21/00;C09K3/00;G01N27/447;G01N33/559 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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