| 摘要 |
<p>The present invention relates to a kinase containing exactly one label in the C helix, (a) wherein said label is a fluorophore, a spin label or an isotope label, (a)(i) wherein said fluorophore and said spin label are covalently bound to a thiol group or side chain amino group of an amino acid residue of said C helix, (a)(ii) wherein said isotope label is a moiety comprising one or more atoms which are isotopes with non-zero nuclear spin, said moiety being covalently bound to a thiol group or side chain amino group of an amino acid residue of said C helix, or said isotope label is an amino acid residue of said C helix, said amino acid residue comprising one or more atoms which are isotopes with non-zero nuclear spin, wherein said isotopes with non-zero nuclear spin are enriched as compared to the natural occurrence thereof.</p> |
