TSG PRIMER TARGET DETECTION

摘要

PURPOSE: A method for detecting nucleic acids using a TSG(target signal generating) primer is provided to amplify a target and a signal and to enable real-time target detection without a complex oligonucleotide. CONSTITUTION: A method for detecting a target nucleic acid from DNA or a nucleic acid mixture using a TSG primer comprises: a step of hybridizing the target nucleic acid sequence with the TSG primer; a step of contacting the resultant with a template-dependent nucleic acid polymerase under conditions for primer elongation; and a step of detecting the signal. [Reference numerals] (AA) Real time signal generation; (BB) TSG primer hybridization and signal generation; (CC) TSG primer; (DD) Hybridized TSG primer; (EE) Target nucleic acid; (FF) Detectable signal indicating the presence of target nucleic acids is acquired by hybridization of TSG primer and target nucleic acid; (GG) 5'→3' nuclease activity-free nucleic acid polymerase; (HH) TSG primer elongation; (II) TSG primer is contained in elongated product and is stabilized.; (JJ) Reporter molecule; (KK) Quencher molecule