发明名称 |
Diagnosis of metal allergy through cytokine release by T-cells in vitro |
摘要 |
An in vitro method for diagnosing a contact allergy in a subject comprises: (i) providing (a) a contact allergen; (b) T-cells from said subject; and (c) a surface to which is immobilized a first specific binding agent capable of specifically binding to a cytokine released from a T-cell in response to said allergen; (ii) contacting said sample with said allergen under conditions which are suitable for (a) release of said cytokine by T cells that have been presensitized in vivo to said allergen; and (b) binding of said cytokine to said first specific binding agent; and (iii) detecting binding of said substance to said first specific binding agent. A kit which enables the method to be carried out is also described. |
申请公布号 |
US8278054(B2) |
申请公布日期 |
2012.10.02 |
申请号 |
US20040471479 |
申请日期 |
2004.01.14 |
申请人 |
JAKOBSON EVA;MABTECH AB |
发明人 |
JAKOBSON EVA |
分类号 |
G01N33/53;G01N33/50;G01N33/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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