METHOD AND APPARATUS FOR PRECISE SELECTION AND EXTRACTION OF A FOCUSED COMPONENT IN ISOELECTRIC FOCUSING PERFORMED IN MICRO-CHANNELS

摘要

An apparatus and method is disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel in fluid communication with each other at a point of intersection. Means are provided selectively moving the pattern of separated zones following cIEF to the intersection and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte out of the extraction channel for collection.