发明名称 |
ISOLATION OF DNA MOLECULES USING MERCAPTO-ARYL LIGANDS |
摘要 |
The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.
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申请公布号 |
CA2444259(C) |
申请公布日期 |
2012.10.02 |
申请号 |
CA20022444259 |
申请日期 |
2002.04.15 |
申请人 |
AMERSHAM BIOSCIENCES AB |
发明人 |
LEMMENS, RAF;NYHAMMAR, TOMAS;BERGLOF, JAN;STADLER, JOACHIM |
分类号 |
C12N15/10;G01N30/06;B01D15/38;B01J20/281;B01J20/283;B01J20/285;B01J20/286;B01J41/00;C12N15/09;C12Q1/68;G01N30/26;G01N30/50;G01N30/80;G01N30/88 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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