| 摘要 |
Provided is a method for making a supplement for cell cultivation media, comprising: (a) centrifuging mammal blood and collecting supernatant; (b) adding platelets activator into the supernatant to obtain activation supernatant; and (c) sterilizing the activation supernatant, wherein platelets activator contains collagen, thrombin and CaCl2, and wherein concentrations of the collagen, thrombin and CaCl2 are in a range selected from the group consisting of: collagen has concentration from 1000 ug/ml to 5000 ug/ml, thrombin has concentration from 5 U/ml to 50 U/ml, and CaCl2 has concentration from 0.1 % to 4.0 %; collagen has concentration from 1000 ug/ml to 5000 ug/ml, thrombin has concentration from 0.1 U/ml to 2.0 U/ml, and CaCl2 has concentration from 0.1 % to 4.0 %; and collagen has concentration from 10 ug/ml to 800 ug/ml, thrombin has concentration from 5 U/ml to 50 U/ml, and CaCl2 has concentration from 0.1 % to 4.0 %.
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