| 摘要 |
<p>The present invention relates to a process for producing a planting material free of PRNSV by in vitro cultures in sour cherry tree variety named Schattenmorelle, wherein a biological material consisting of a meristem of a dimension of 0.1...0.2 mm, obtained from buds located on annual branchlets, is cultivated on a growth medium for the differentiation phase, said growth medium comprising the Murashige-Skoog components in the following amounts: 80...110 ml/l from the stock solution of macro-elements with a concentration of 10x, 8...12 ml/l from the stock solution of micro-elements with a concentration of 100x, 8...12 ml/l from the stock solution of vitamins with a concentration of 100x and a hormonal balance represented by 0.05...0.2 mg/l of gibberellic acid, 0.01...0.03 mg/l of indolylbutyric acid and 0.10.5 mg/l of 6-benzylaminopurine.</p> |
