摘要 |
A laser microscope that efficiently performs 3D imaging irrespective of the shape of a specimen, includes an area segmenting section that segments an observation range of a specimen in the direction perpendicular to the optical axis of an objective lens into many areas; a surface-position storing section that stores motorized stage positions in association with the specimen surface positions, for the many areas; a surface-shape estimating section that estimates the specimen surface shape from the motorized stage positions and the specimen surface positions for the many areas; a z-scanning condition determining section that determines the specimen surface positions at desired positions of the motorized stage from the specimen surface shape; and a light detecting section that detects light from the specimen over certain ranges specified with reference to the specimen surface positions, in the optical axis direction of the objective lens.
|