FUSION PROTEIN, FUSION PROTEIN-PRODUCING STRAIN OF ESCHERICHIA COLI BACTERIA AND A METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2B FROM SAID FUSION PROTEIN

摘要

The invention relates to microbiology and the medical industry and also to genetic engineering and biotechnology. By engineering recombinant plasmid DNA containing the structural gene of human interferon alpha-2b under the control of a regulated promoter, a strain of Escherichia coli bacteria is produced which provides the synthesis of the precursor protein of mature methionine-free human interferon alpha-2b in an insoluble form. In the precursor protein, the sequence of mature methionine-free interferon alpha-2b is fused with the sequence of the SUMO protein (SMT3) of the yeast Saccharomyces cerevisiae. A strain of Escherichia coli bacteria is produced which provides the biosynthesis of ULP275 protease for the enzymatic processing of the precursor protein of methionine-free interferon. A method is proposed for producing methionine-free interferon, which involves the joint implementation of the processes of renaturation, disulphide bond formation and enzymatic isolation of methionine-free interferon alpha-2b from the precursor protein under the effect of ULP275 protease.