发明名称 |
A YEAST RECOMBINANT CELL CAPABLE OF PRODUCING GDP-FUCOSE |
摘要 |
A yeast strain expressing a bi-functional fucokinase/GDP-L-fucose pyrophosphorylase enzyme and capable of producing GDP-L-fucose in vivo is provided. Also provided are yeast cells which express a GDP-L-fucose transporter and /or a fucosyl transferase with the bi-functional enzyme. In addition, the said yeast contains one or more expression cassettes for fusion proteins of heterologous glycosylation pathway and an ER/Golgi retention sequence. Finally, the invention also provides a method for producing recombinant target glycoproteins. |
申请公布号 |
WO2012127045(A1) |
申请公布日期 |
2012.09.27 |
申请号 |
WO2012EP55245 |
申请日期 |
2012.03.23 |
申请人 |
GLYCODE;JAVAUD, CHRISTOPHE;ARICO, CHRISTELLE;BONNET, CHRISTINE |
发明人 |
JAVAUD, CHRISTOPHE;ARICO, CHRISTELLE;BONNET, CHRISTINE |
分类号 |
C12N1/16;C12N9/10;C12N9/12 |
主分类号 |
C12N1/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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