METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS
摘要
Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
申请公布号
CA2831144(A1)
申请公布日期
2012.09.27
申请号
CA20122831144
申请日期
2012.03.22
申请人
PIONEER HI-BRED INTERNATIONAL, INC.;E.I. DU PONT DE NEMOURS AND COMPANY
发明人
LASSNER, MICHAEL;JANTZ, DEREK;SMITH, JAMES JEFFERSON;CIGAN, MARK;FALCO, CARL;GAO, HUIRONG;LI, ZHONGSEN;LIU, ZHAN-BIN;SVITASHEV, SERGEI