发明名称 |
Method for creating polynucleotide and polypeptide sequences |
摘要 |
<p>The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.</p> |
申请公布号 |
EP1036198(B1) |
申请公布日期 |
2012.09.26 |
申请号 |
EP19980960709 |
申请日期 |
1998.12.04 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
ARNOLD, FRANCES;SHAO, ZHIXIN;VOLKOV, ALEXANDER |
分类号 |
C12N15/09;C12Q1/68;C07K14/435;C12N15/10;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|