发明名称 |
DELIVERY OF NUCLEIC ACIDS INTO EUKARYOTIC GENOMES USING IN VITRO ASSEMBLED MU TRANSPOSITION COMPLEXES |
摘要 |
The present invention relates to genetic engineering and especially to the use of DNA transposition complex of bacteriophage Mu. In particular, the invention provides a gene transfer system for eukaryotic cells, wherein in vitro assembled Mu transposition complexes are introduced into a target cell and subsequently transposition into a cellular nucleic acid occurs. The invention further provides a kit for producing insertional mutations into the genomes of eukaryotic cells. The kit can be used, e.g., to generate insertional mutant libraries.
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申请公布号 |
CA2522438(C) |
申请公布日期 |
2012.09.25 |
申请号 |
CA20042522438 |
申请日期 |
2004.04.14 |
申请人 |
FINNZYMES OY |
发明人 |
SAVILAHTI, HARRI;FRILANDER, MIKKO;MENG, XIAOJUAN;PAATERO, ANJA;PAJUNEN, MARIA;TURAKAINEN, HILKKA |
分类号 |
C12N15/90;C12N9/22;C12N15/10;C12N15/79 |
主分类号 |
C12N15/90 |
代理机构 |
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主权项 |
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地址 |
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