METHOD OF PROVIDING INFORMATION FOR DIAGNOSIS OF TUBERCULOSIS USING QUANTITATIVE REALTIME PCR AND DIAGNOSTIC KIT COMPRISING THEREOF

摘要

PURPOSE: A kit for diagnosing tuberculosis by real-time RT-PCR is provided to enable test using a small amount of blood. CONSTITUTION: A method for providing information for diagnosing tuberculosis comprises: a step of collecting blood from a patient who is suspicious for tuberculosis and treating tuberculosis bacillus-specific antigen to the blood; a step of isolating full length RNA from the blood; a step of synthesizing cDNA from the full length RNA; a step of performing real-time RT-PCR; and a step of measuring IFN-gamma mRNA expression level. The tuberculosis bacillus-specific antigen is ESAT-6, CFP-10 or TB7.7. A primer for amplifying IFN-gamma contains a primer pair of sequence numbers 1 and 2. [Reference numerals] (AA) Step of collecting blood from a patient who is suspected to have tuberculosis and treating with tuberculosis bacillus-specific antigen; (BB) Step of isolating full-length RNA from blood treated with tuberculosis bacillus-specific antigen; (CC) Step of synthesizing cDNA from the isolated full length RNA; (DD) Step of real time RT-PCR of synthesized cDNA using first primer pair which is able to amplify IFN-γ, first probe which binds to amplified site, second primer pair which is able to amplify control group gene to compare with IFN-γexpression level, and second probe which binds to amplified control group gene; (EE) Step of measuring mRNA expression level of the amplified IFN-γand mRNA expression level of control group