发明名称 |
METHOD FOR PREPARING SUBSTRATE FOR ARRAYING ANIMAL CELLS THEREON AND METHOD FOR PREPARING SUBSTRATE HAVING ANIMAL CELLS ARRAYED THEREON |
摘要 |
<p>A new means of separately arranging individual animal cells on a substrate surface is provided. A method for preparing a substrate for arranging animal cells in an array, comprising steps (1) to (3): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; and (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with the extracellular matrix adhered to the adsorption surfaces thereof. A method for preparing a substrate on which animal cells have been arranged in an array, comprising steps (1) to (4): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with extracellular matrix adhered to the adsorption surfaces thereof; and (4) culturing the animal cells on the surface of the substrate obtained in (3) to obtain a substrate on which the animal cells have adhered to the adsorption surface.</p> |
申请公布号 |
EP2497824(A1) |
申请公布日期 |
2012.09.12 |
申请号 |
EP20100826846 |
申请日期 |
2010.10.29 |
申请人 |
JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY |
发明人 |
KOYAMA, SUMIHIRO |
分类号 |
C12N5/071;C12M1/40;C12M3/00;G01N37/00 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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