| 发明名称 |
METHODS FOR SITE-DIRECTED MUTAGENESIS AND TARGETED RANDOMIZATION |
| 摘要 |
The present invention provides methods and compositions for the construction and direct transformation of site-saturation libraries into Bacillus. This method avoids the need for the use of intermediate hosts, such as E. coli for the development of Bacillus strains suitable for the production of proteins.
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| 申请公布号 |
CA2513227(C) |
申请公布日期 |
2012.09.11 |
| 申请号 |
CA20042513227 |
申请日期 |
2004.01.16 |
| 申请人 |
GENENCOR INTERNATIONAL INC. |
发明人 |
LEEFLANG, CHRIS;VAN DER KLEIJ, WILHELMUS A. H. |
| 分类号 |
C12N15/75;A61K;C12N15/00;C12N15/09;C12N15/10;C12N15/63;C12N15/70;C12N15/74;C12N15/85;C12N15/87 |
主分类号 |
C12N15/75 |
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