INDUCTION OF IMMUNE RESPONSE BY A REPLICATION-DEFECTIVE VENEZUELAN EQUINE ENCEPHALITIS-SINDBIS CHIMERIC VIRUS REPLICON PARTICLE ENCODING AN ANTIGEN

摘要

A replication-defective alphavirus replicon particle containing an alphavirus vector construct comprising a heterologous polynucleotide encoding at least one first antigen or modified form thereof, said first antigen or modified form thereof being capable of stimulating an immune response in a subject when administered intranasally, wherein said replication-defective alphavirus replicon particle is a chimeric alphavirus particle containing a Venezuelan Equine Encephalitis (VEE) virus vector construct packaged with Sindbis (SIN) virus envelope glycoproteins, for use in treating a mammalian subject by generating an immune response in the subject by intranasal administration. The at least one first antigen may be an antigen from a pathogen and induces an immune response to the pathogen. Additional antigens or immunostimulatory factors may also be encoded by the chimeric replicon particle, or co-administered as a polypeptide.