发明名称 |
Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
摘要 |
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
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申请公布号 |
US8263365(B2) |
申请公布日期 |
2012.09.11 |
申请号 |
US201113008392 |
申请日期 |
2011.01.18 |
申请人 |
WILLIAMS PETER;TAYLOR THOMAS J.;WILLIAMS DANIEL J. B.;GOULD IAN;HAYES MARK A.;ARIZONA BOARD OF REGENTS |
发明人 |
WILLIAMS PETER;TAYLOR THOMAS J.;WILLIAMS DANIEL J. B.;GOULD IAN;HAYES MARK A. |
分类号 |
C12P19/34;C07H21/00;C07H21/02;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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