ASSAY FOR DETECTING CLOSELY- RELATED SEROTYPES OF HUMAN PAPILLOMAVIRUS (HPV).
摘要
<p>A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.</p>
申请公布号
MX2012009406(A)
申请公布日期
2012.09.07
申请号
MX20120009406
申请日期
2011.02.16
申请人
BECTON, DICKINSON AND COMPANY.*
发明人
CHEN, CHI;PECK, HUGH, J.;PORTER, MICHAEL;RICHART, GREGORY, A.;MC MILLIAN, RAY, A.