发明名称 |
METHOD FOR PREPARATION OF PROLIFERATABLE ANIMAL CELLS |
摘要 |
<p>A method for preparing animal cells with good adhesion and proliferation properties which are capable of proliferation is provided. the method permits the detachment of cultured cells without damaging the cells. A method for preparing a sheet of animal cells such as skin cells which are capable of proliferation is also provided. A method for preparing animal cells capable of proliferation, comprising the steps of (1) culturing animal cells on a substrate at least one portion of which is an electrode, and (2) applying a high-frequency wave potential to the electrode to detach the cells that have adhered to the substrate surface through culturing. The high-frequency wave potential is of a frequency falling within a range of 1 KHz to 10 MHz with a potential falling within a range of ± 1.0 V (vs. Ag/AgCl) or less. The culture medium during separation does not contain calcium or magnesium.</p> |
申请公布号 |
EP2495309(A1) |
申请公布日期 |
2012.09.05 |
申请号 |
EP20100826847 |
申请日期 |
2010.10.29 |
申请人 |
JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY |
发明人 |
KOYAMA, SUMIHIRO |
分类号 |
C12N5/071;A61L27/00;C12M3/00;C12N5/07 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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