Isolated gene of Corynebacterium glutamicum, recombinant coryneform bacteria and process for preparation of L-lysine or L-threonine

摘要

An isolated gene of Corynebacterium glutamicum (zwf gene) obtainable by PCR from C. glutamicum ATCC 13032 by means of the oligonucleotide primer: zwf-forward, SEQ ID NO:10: 5'-TCG ACG CGG TTC TGG AGC AG-3', zwf-reverse, SEQ ID NO:11: 5'-CTA AAT TAT GGC CTG CGC CAG-3' encoding a polypeptide having the activity of glucose-6-phosphate dehydrogenase, wherein said polypeptide contains the N-terminal amino acid sequence SEQ ID NO:9 Ser Thr Asn Thr Pro Ser Ser Trp Thr Asn Pro Arg Asp. L-lysine or L-threonine producing recombinant coryneform bacteria wherein said bacteria are transformed by said gene. A process for the preparation of L-lysine or L-threonine by fermentation of coryneform bacteria, which comprises carrying out the following steps: a) fermentation of said L-amino acid-producing bacteria in which at least the zwf gene encoding glucose-6-phosphate dehydrogenase is amplified, b) concentration of said L-amino acid in the medium or in the cells of the bacteria and c) isolation of the L-amino acid produced.