NOVEL 12 ALPHA-HYDROXYSTEROID DEHYDROGENASES, PRODUCTION AND USE THEREOF

摘要

Disclosed is a 12alpha-hydroxysteroid dehydrogenase obtained by heterologous expression of at least one 12a-HSDH-encoding nucleic acid sequences in a non-pathogenic microorganism, wherein the 12alpha-hydroxysteroid dehydrogenase comprises one of the following N-terminal sequences: MDFIDFKEMGRMGIFDGKVAIITGGGKAKSIGYGIAVAYAK (SEQ ID NO: 6) and MDFIDFKEMGRMGI (SEQ ID NO: 7).