Recombinant allergen, the use of the recombinant allergen, a composition containing two or more variants of recombinant mutant allergens, the use of that composition, a pharmaceutical composition containing the recombinant allergen or the said composition, the use of the recombinant allergen, composition or pharmaceutical composition, a method for preparing the pharmaceutical composition, a method for preparing the recombinant allergen, a method for preparing a library of the recombinant allergen, DNA sequence encoding the recombinant allergen, expression vector comprising that DNA, host cell containing that expression vector, a method for obtaining recombinant mutant allergen, and diagnostic method to assess the suitability, safety or outcome of the therapy

摘要

Novel recombinant allergens with multiple mutations and reduced IgE binding affinity are disclosed. The allergens are mutants of naturally occurring allergens. The overall alpha-carbon backbone tertiary structure is essentially preserved. Also disclosed is a method for preparing such recombinant allergens as well as uses thereof.