发明名称 Targeted Chromosomal Mutagenesis Using Zinc Finger Nucleases
摘要 The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present invention provides numerous improvements over previous mutagenesis methods, such advantages include that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA into host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.
申请公布号 US2012219959(A1) 申请公布日期 2012.08.30
申请号 US201113314490 申请日期 2011.12.08
申请人 CARROLL DANA;GOLIC MARY M.;BIBIKOVA MARINA;RREWS GARY;GOLIC KENT G. 发明人 CARROLL DANA;GOLIC MARY M.;BIBIKOVA MARINA;RREWS GARY;GOLIC KENT G.
分类号 C12N15/09;C12N15/82;A01K67/033;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/22;C12N15/01;C12N15/74;C12N15/79;C12N15/85;C12N15/90;C12Q1/68 主分类号 C12N15/09
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