METHOD FOR CONCENTRATING CELLS THAT ARE GENETICALLY ALTERED BY NUCLEASES

摘要

The present invention relates to a reporter composition and to a method for composing the reporter for selecting or concentrating cells in which a specific nucleotide sequence in cells is cut by a specific nuclease, or the nucleotide sequence is altered by the cutting. The present invention also relates to host cells comprising the reporter composition, and to a system for monitoring nuclease activity. The reporter system of the present invention is simple and non-invasive, and enables the efficient concentration of genetically altered cells. Accordingly, the method of the present invention may promote the use of nucleases not only in gene treatment and genetic engineering, but also in basic research.