发明名称 |
COMPOSITION FOR HOT-START PCR COMPRISING BLOCKING OLIGONUCLEOTIDE |
摘要 |
The present invention relates to a composition for hot-start PCR, more precisely a composition for hot-start PCR characteristically prepared by adding the blocking oligonucleotide having the hydroxyl group at 3'-end blocked and the nucleotide sequence complementary to the primer to the conventional PCR composition containing reaction buffer, MgCl2, 4 kinds of dNTP, and DNA polymerase. The composition for hot-start PCR of the present invention can give more authentic PCR results by inhibiting smear band of PCR amplification product caused by non-specific reaction or primer-dimer formation at low temperature since the blocking oligonucleotide included therein can be dissociated at the lower temperature than melting temperature of the primer. |
申请公布号 |
WO2012115464(A2) |
申请公布日期 |
2012.08.30 |
申请号 |
WO2012KR01387 |
申请日期 |
2012.02.23 |
申请人 |
BIONEER CORPORATION;LEE, JUN HEE;CHOI, SO RA;KIM, NAM IL;PARK, HAN OH |
发明人 |
LEE, JUN HEE;CHOI, SO RA;KIM, NAM IL;PARK, HAN OH |
分类号 |
C12Q1/68;C12N15/11;C12Q1/48 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|