发明名称 |
METHODS FOR MONITORING CELLULAR STATES AND FOR IMMORTALIZING MESENCHYMAL STEM CELL |
摘要 |
We describe a method of monitoring the state of a cell, the method comprising establishing, for a selected microRNA (miRNA) species secreted by the cell, a ratio of: (a) a precursor form of the miRNA species (pre-miRNA); to (b) a mature form of the miRNA species (mature miRNA); in which the pre- to mature miRNA ratio so established is indicative of the state of the cell. We also describe a method comprising the steps of: (a) providing a mesenchymal stem cell (MSC); and (b) introducing an oncogene into the mesenchymal stem cell to thereby transform it; in which the transformed mesenchymal stem cell does not secrete a gene product of the oncogene into a medium in which it is grown. |
申请公布号 |
US2012219632(A1) |
申请公布日期 |
2012.08.30 |
申请号 |
US201013505622 |
申请日期 |
2010.11.02 |
申请人 |
LIM SAI KIANG;AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH |
发明人 |
LIM SAI KIANG |
分类号 |
C12Q1/68;A61K35/12;A61K48/00;A61P35/00;C07H21/02;C12N5/0775;C12N15/87;C12Q1/02;C40B30/04;C40B40/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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