IMMUNOENZYME TEST-SYSTEM FOR QUALITATIVE AND SEMI-QUANTITATIVE DETERMINATION OF IGG CLASS ANTIBODIES TO HUMAN CYTOMEGALOVIRUS (DIA-CMV-IGG-U)

摘要

An immunoenzyme test-system for qualitative and semi-quantitative determination of IgG class antibodies to human cytomegalovirus "DIA-CMV-u", wherein recombinant anti-genes are sorbed to polystyrene tray, and as conjugate one uses antibodies against human IgG class immunoglobulins. Recombinant polypeptides antigens p150-HIS-6 and p28 of cytomegalovirus sorbed to polystyrene are used as immunosorbent. Peroxidase conjugate of monoclonal antibodies to human class G immunoglobulins is used that is detected by means of one-component solution of chromogen, and for estimation of change of level of antibodies in dynamics result of analysis is expressed in DIA Units (DU).