发明名称 |
Method for production of live smallpox vaccine |
摘要 |
<p>Disclosed are: a safer live smallpox vaccine which contains an MSP strain in a reduced amount; and a method for production of the live smallpox vaccine. Specifically, disclosed is a method for production of a live smallpox vaccine, comprising the steps of: inoculating a master seed solution of attenuated vaccinia virus to a proper number of groups (1 to n; n is an integer) of rabbit primary kidney cells and culturing the cells; inoculating a part of the culture of each of the group to a RK-13 cell and a Vero cell, culturing the cells, and selecting and collecting a culture of a group which causes the formation of a plaque in a RK-13 cell but does not cause the formation of any plaque in a Vero cell; and preparing a vaccine stock solution using the culture (a working seed solution). Also specifically disclosed is a live smallpox vaccine prepared by the method.</p> |
申请公布号 |
AU2007282612(B2) |
申请公布日期 |
2012.08.23 |
申请号 |
AU20070282612 |
申请日期 |
2007.08.06 |
申请人 |
JURIDICAL FOUNDATION THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE;JAPAN AS REPRESENTED BY DIRECTOR-GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES |
发明人 |
OHKUMA, KUNIO;KANEHARA, TOMOMI;KURANAGA, MASAHIKO;YOKOTE, HIROYUKI;MORIKAWA, SHIGERU |
分类号 |
A61K39/285 |
主分类号 |
A61K39/285 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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