| 摘要 |
<p>The invention relates to a detection system for detecting a signal in a sandwich ELISA assay, the detection system comprising an amplification system and a sensing apparatus, the amplification system comprising: a binding molecule releasably attached to a first releasing molecule; and a plurality of manipulating agents immobilised on a substrate, and wherein the sensing apparatus comprises: a substrate; an elongate molecule attached at one end to the substrate; a magnetic bead attached to the other end of the elongate molecule; an element to produce a magnetic field; and an electronic sensor to detect movement of the magnetic bead, wherein the element produces a magnetic field which exerts a force on the magnetic bead to stretch the elongate molecule into an extended form, and wherein the sensor detects movement of the magnetic bead resulting from movement of the elongate molecule, wherein, in use, the first releasing molecule is released from the binding molecule by a cleaving agent, wherein, once released, the first releasing molecule causes a plurality of manipulating agents to be released from the substrate, wherein, once released, the manipulating agents interact with the elongate molecule in the sensing apparatus and cause movement of the elongate molecule, and wherein the movement of the elongate molecule causes movement of the magnetic bead which is detected by the electronic sensor.</p> |
