| 摘要 |
This invention relates to methods for the generation and subsequent validation of vascular bed-specific endothelial cells with specific genetic and functional signatures starting from cellular precursors and use thereof. These vascular bed-specific endothelial cells with specific genetic and functional signatures from cellular precursors of the endothelial lineage of the present invention are in a particular embodiment of present invention used for the generation of in vitro bioengineered tissue culture equivalents. These in vitro bioengineered tissue culture equivalents are in an additional embodiment used for drug toxicity testing or for the generation of bioengineered vessel conduits for in vivo transplantation. In yet another embodiment these cells generated by present invention are used for treatment of vascular bed-specific diseases affecting the brain (e.g., stroke), the liver (e.g., sinusoidal obstruction syndrome), the heart (myocardial ischemia), the extremities (e.g., peripheral vascular disease), amongst others. |
