发明名称 METHOD FOR ASSAYING CHOLESTEROL IN ApoE-CONTAINING HDL
摘要 This invention provides a method for separately or simultaneously quantifying cholesterol in a total amount of HDL-C, cholesterol in an HDL subfraction of apoE-containing HDL-C, and cholesterol in an HDL subfraction of apoE-deficient HDL-C. The method comprises enzymatically and separately quantifying cholesterol in apoE-containing HDL and cholesterol in apoE-deficient HDL by adding a surfactant selected from the group consisting of a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 0.7 to less than 1.3, a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of less than 0.7, and a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 1.3 or more to a test sample, allowing cholesterol esterase and cholesterol oxidase to react therewith, and quantifying the hydrogen peroxide generated.
申请公布号 US2012208219(A1) 申请公布日期 2012.08.16
申请号 US201013503924 申请日期 2010.10.26
申请人 ITOH YASUKI;CHIBA HITOSHI;NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSIT;DENKA SEIKEN CO., LTD. 发明人 ITOH YASUKI;CHIBA HITOSHI
分类号 C12Q1/60 主分类号 C12Q1/60
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