A non-invasive gender determination method and kits for accomplishing the same are disclosed herein. The present invention is based on difference in testosterone levels in the maternal body fluids of females carrying male fetuses relative to that of females carrying a female fetus. The present invention does not need specialized equipment and can quickly and rapidly detect the presence of testosterone in maternal urine or serum and thus indicate fetal gender. The kits and methods disclosed herein are useful for breeders of single-fetus mammals, veterinarians, and interested parents.
