发明名称 |
METHOD AND KIT FOR DETECTING FOLTATE |
摘要 |
A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography. |
申请公布号 |
US2012208220(A1) |
申请公布日期 |
2012.08.16 |
申请号 |
US201213372107 |
申请日期 |
2012.02.13 |
申请人 |
FU TZU-FUN;KAO TSENG-TING;NATIONAL CHENG KUNG UNIVERSITY |
发明人 |
FU TZU-FUN;KAO TSENG-TING |
分类号 |
G01N30/06 |
主分类号 |
G01N30/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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