发明名称 |
NOVEL DNA CAPABLE OF BEING AMPLIFIED BY PCR WITH HIGH SELECTIVITY AND HIGH EFFICIENCY |
摘要 |
<p>The present invention relates to unnatural base pairs of Ds (a 5-amino-7-(2-thienyl)-3H-imidazo[4,5-b] pyridine-3-yl group) and a Pa derivative (a 2-nitro-1H-pyrrole-1-yl group bearing a substituent having a À-electron system attached at position 4) that can be replicated with high selectivity/high efficiency, and methods for replicating nucleic acids containing the unnatural base pairs. The present invention also relates to methods for incorporating an unnatural base bearing a functional substituent attached thereto into DNA by a nucleic acid replication reaction. The present invention also relates to methods for replicating and selectively collecting a nucleic acid containing an unnatural base pair from a nucleic acid pool. The present invention also relates to methods for determining a sequence of natural bases in the proximity of an unnatural base in DNA for achieving highly efficient and highly selective replication of a nucleic acid containing the unnatural base.</p> |
申请公布号 |
EP2275538(A4) |
申请公布日期 |
2012.08.15 |
申请号 |
EP20090728695 |
申请日期 |
2009.03.31 |
申请人 |
RIKEN;TAGCYX BIOTECHNOLOGIES |
发明人 |
HIRAO, ICHIRO;HIRAO, MICHIKO;YOKOYAMA, SHIGEYUKI |
分类号 |
C12N15/09;A61K31/7115;C07H21/04;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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