| 摘要 |
Disclosed is a DNA construct that is useful for efficient production of recombinant CHO cells useful for the production of target proteins. The DNA construct is a constract comprising, from a 5' end toward a 3' end, a first homologous DNA fragment, a target protein gene, and a second homologous DNA fragment. The first and second homologous DNA fragments have homology allowing for homologous recombination with a part of a hypoxanthine-phosphoribosyltransferase enzyme ( hprt ) locus in a CHO cell genome and have a chain length of not less than 1 kbp.
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