发明名称 |
Modified polypeptides stabilized in a desired conformation and methods for producing same |
摘要 |
The present invention provides a method for stabilizing a protein in a desired conformation by introducing at least one disulfide bond into the polypeptide. Computational design is used to identify positions where crysteine residues can be introduced to form a disulfide bond in only one protein conformation, and therefore lock the protein in a given conformation. Accordingly, antibody and small molecule therapeutics are selected that are specific for the desired protein conformation. The invention also provides modified integrin I-domain polypeptides that are stabilized in a desired conformation. The invention further provides screening assays and therapeutic methods utilizing the modified integrin I-domains of the invention.
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申请公布号 |
US8241627(B2) |
申请公布日期 |
2012.08.14 |
申请号 |
US201113165873 |
申请日期 |
2011.06.22 |
申请人 |
SPRINGER TIMOTHY A.;SHIMAOKA MOTOMU;LU CHAFEN;THE CENTER FOR BLOOD RESEARCH, INC. |
发明人 |
SPRINGER TIMOTHY A.;SHIMAOKA MOTOMU;LU CHAFEN |
分类号 |
A01K67/027;A61K39/395;A61K35/76;A61K38/00;A61K39/00;A61K45/00;A61K48/00;A61P1/04;A61P9/00;A61P9/10;A61P11/00;A61P11/06;A61P13/12;A61P17/00;A61P17/02;A61P17/06;A61P19/02;A61P25/04;A61P29/00;A61P37/02;C07K14/705;C07K16/28;C12N15/09;C12N15/12;G01N33/15;G01N33/50;G01N33/53;G01N33/566 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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