METHOD OF EXTRACTING PLASMINOGEN OR PLASMIN FROM MIXTURE IN PRESENCE OF FIBRINOGEN

摘要

FIELD: chemistry. ^ SUBSTANCE: invention relates to biotechnology and can be used to extract plasminogen or plasmin. A mixture containing fibrinogen and plasminogen or plasmin is deposited onto a chromatographic column containing an insoluble affinity chromatography matrix which is covalently cross-linked with tranexamic acid. Plasminogen or plasmin is then eluted from the affinity chromatography matrix with an aqueous solution containing a sufficient amount of a ligand which competes with plasmin or plasminogen for binding sites of said matrix which is bonded with tranexamic acid. Tranexamic acid is covalently bonded with said matrix through a linker which lies between the matrix and tranexamic acid and whose length is more than three carbon atoms. ^ EFFECT: invention increases efficiency of extracting plasminogen or plasmin from a mixture in the presence of fibrinogen. ^ 10 cl, 1 dwg, 13 tbl, 6 ex