COMPOSITIONS AND METHODS FOR SEQUENCING DNA

摘要

Provided are compositions and methods for sequencing a target polynucleotide that has been used to generate an elongated polynucleotide having a plurality of segments, each segment corresponding to one of the nucleic acid residues of the target polynucleotide and having a sequence motif. The composition includes a first oligonucleotide for specifically hybridizing to any segment of the elongated polynucleotide having the first sequence motif, a second oligonucleotide for specifically hybridizing to any segment of the elongated polynucleotide having the second sequence motif, a third oligonucleotide for specifically hybridizing to any segment of the elongated polynucleotide having the third sequence motif; and a fourth oligonucleotide for specifically hybridizing to any segment of the elongated polynucleotide having the fourth sequence motif. The first, second, third, and fourth oligonucleotides each independently are complexed with a first, second, third, and fourth nanoparticle, respectively. The nanoparticles are distinguishable from one another using scanning electron microscopy.